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The power of hypothesis: although a powerful statistical method, hypothesis testing can lead to false conclusions if applied incorrectly.(Statistics Essentials)

Publication: Biopharm International
Publication Date: 01-JUN-08
Format: Online
Delivery: Immediate Online Access

Article Excerpt
This article is the second in a four part series on essential statistical techniques for any scientist or engineer working in the biotechnology field. This installment presents statistical methods for comparing sample means, including how to establish the correct sample size for testing these...

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...differences. The difference between one-sample, two-sample, and z-test also are explored.

HYPOTHESIS TESTING

In hypothesis testing, we must state the assumed value of the population parameter called the null hypothesis. The goal of hypothesis testing is to verify if the sample data is part of the population of interest. You either have sufficient evidence to accept the null hypothesis or reject it--you do not prove it. The significance level or p-value indicates the likelihood that the sample comes from the population of interest. Statisticians usually use a p-value of 0.05 as the cutoff for statistical significance. In other words, a p-value less than 0.05 is sufficient evidence to reject the null hypothesis. Typically, the null hypothesis is a statement about the value of the population parameter. For example, [micro] = 100 versus [micro] [not equal to] 100. A one-sided test means we are testing the null hypothesis of either less than or greater than. A two-sided test means we are testing the null hypothesis of less than and greater than.

ONE SAMPLE T-TEST

The one-sample t-test is used to compare a sample mean to a hypothesized population mean. The hypothesis can be either a one-sided or two-sided test. Usually,...

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