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Mississippi Academy of Sciences seventy-first annual meeting--program errata.

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AGRICULTURE AND PLANT SCIENCE

FRIDAY AFTERNOON, FEBRUARY 23

New Posters, Friday, 3:00p.

CHARACTERIZATION OF OXIDATION PRODUCTS FROM CATFISH OIL AND BIODIESEL

Supanee Danviriyakul (1), Ashli E. Brown (2*), William E. Holmes (3), Elizabeth C. Rogers (3), and Juan L. Silva (2), (1) Chandrakasem Rajabhat University. Bangkok, Thailand; (2) Mississippi State University, and (3) Mississippi State Chemical Laboratory, Mississippi State, MS 39762.

The stability of biodiesel depends on the quality of the feedstock, transesterification process, environmental conditions, and other factors. Oxidation of biodiesel can lead to lower engine performance and shorter life. Oil breakdown can occur through hydrolysis of triglycerides and through oxidation of fatty acid methyl esters (FAMEs), resulting in low molecular weight FAs and/or polymerized molecules of very high MW. Polymerization can result in increased viscosity of the biodiesel and failure to meet ASTM standards. Catfish oil was trasesterified with excess methanol and sodium hydroxide and refined. The resulting product was heated to 80 and 110[degrees]C, with and without air. The oxidation products were analyzed using GC/MS and LC/MS. These highly selective analytical techniques allow identification and monitoring of these products. Samples stored without available oxygen did not produce significant ROOH or conjugated dienes, and the acid number remained low. When the samples were stored open to air, ROOH and conjugated dienes both increased. The acid number and viscosity increased more in samples exposed to air than for samples not exposed to air

UTILIZING ZEBRAFISH AS A MODEL FOR FISH DISEASES.

L. Petrie-Hanson, P. Khosravi, C.M. Hohn and L. Hanson. Mississippi State University, Mississippi State, MS 39762.

Aquaculture, or the production and rearing of aquatic animal species, is rapidly growing throughout the world to replace dwindling natural resources. Channel catfish production is the largest aquaculture enterprise in the United States. The leading cause of loss in channel catfish (Ictalurus punctatus) production is disease, with very high losses occurring at the fry stocking to fingerling stage. The leading cause of fry/fingerling loss is Enteric Septicemia of Catfish (ESC), with 57.8% of fry/fingerling producers reporting losses. Providing broad protection against multiple pathogens at an early developmental stage is especially critical in extensive aquaculture because very young fish are placed in extremely vulnerable situations. Research investigating disease progression in channel catfish fry is difficult because channel catfish take 3 years to mature, and spawn once a year, so the availability of fry for developmental immunology studies is limited. Additionally, the genome of this species has not been sequenced, and micro array reagents are limited. These factors negatively impact developmental immunology and infectious disease research of this commercially important fish. Alternatively, zebrafish reproduce by three months of age, spawn weekly, are hardy and easy to maintain. The zebrafish genome has recently been sequenced; so characterizing the interaction of zebrafish and pathogens can be more readily investigated at the molecular level. We are investigating disease progression of several fish pathogens utilizing the zebrafish model.

CELLULAR, MOLECULAR AND DEVELOPMENTAL BIOLOGY

THURSDAY AFTERNOON, FEBRUARY 22

Moved Lecture, 2:00p. Moved to Health Sciences, Poster, Friday, 9:30a.

DELETION AND EPITOPE-TAGGING OF CELL CYCLE GENES USING UNCLONED PCR FUSION PRODUCTS AND HOMOLOGOUS RECOMBINATION IN ASPERGILLUS NIDULANS

Moved Lecture, 3:30p. Moved to Lecture, Friday, 10:15a.

EXPRESSION ANALYSIS OF THE MOLD-SPECIFIC M46 GENE IN FIVE STRAINS OF THE PATHOGENIC...

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