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Section V: Biomedical Sciences tapley hall, room 119 Francis Eko, presiding.

Article Excerpt
9:00 REPLACEMENT OF MEMBRANE CHOLESTEROL DURING PHYTOSTEROL SUPPLEMENTATION IN PROSTATE CANCER CELLS**, Wambui S. Wandu*, Godwin O. Ifere and Godwin A. Ananaba, Department of Biological Sciences, Clark Atlanta University, Atlanta, GA 30314. Enhancement of cellular phytosterol levels may improve its chemotherapeutic potential in prostate cancer cells. This is due to the suggestion that phytsoterols may replace membrane cholesterol, which is known to promote cell proliferation. There is little information that supports phytosterol replacement of membrane cholesterol. We therefore hypothesize that cutback of in vitro cholesterol absorption by supplementation of prostate cancer cells with phytosterols may result in membrane cholesterol depletion and increased phytosterol buildup. In vitro cultures of prostate cancer cell lines PC-3 were supplemented with cholesterol and phytsoterol, facilitated by [beta]-cyclodextrin (as vehicle). After 72 h of incubation at 37[degrees]C, the cells were harvested and their membranes isolated by density gradient centrifugation. After extraction of membrane lipids with chloroform /methanol, cholesterol was estimated by a sensitive enzymatic assay. Our results indicate a minimized level of cholesterol in membranes from phytosterol-treated cells. We conclude that phytosterol treatment might diminish membrane cholesterol and contribute to the success of prostate cancer chemotherapy.

9:15 PLASMA INTERLEUKIN-1[beta] CONCENTRATION PREDICTS RISK...

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